Studies of cellular toxicity of unconjugated bilirubin in kernicteric brain.

Studies of cellular toxicity of unconjugated bilirubin in kernicteric brain.

Intracerebral deposition of unconjugated bilirubin is believed to be primarily responsible for the neurological manifestations of kernicterus1 (1). Numerous in vitro studies have been carried out on the effect of unconjugated bilirubin on metabolic activity of liver and brain mitochondria (2), brain homogenates (3), cells grown in tissue culture (4, 5), and unicellular organisms (6). The majori...

Kinetics of hepatic uptake of unconjugated bilirubin.

1. The uptake of bilirubin was studied in the perfused rat liver by a multiple-indicator dilution technique employing the three-compartment model of Goresky. 2. The kinetics of hepatic bilirubin uptake could be described by the Michaelis-Menten equation. 3. The maximal uptake velocity (V max.) and the apparent half-saturation constant (Km) were 4-4 +/- 0-5 nmol s-1 g-1 of liver and 58 +/- 16 nm...

Interactions of unconjugated bilirubin with bile salts.

The rate of peroxidation of unbound, unconjugated bilirubin (UCB) was used to assess the interactions of UCB with four taurine-conjugated bile salts at pH 8.2, 37 degrees C, and an ionic strength of 0.15. Each of the four structurally different bile salts markedly decreased the rate of peroxidation of UCB in the presence of horseradish peroxidase (HRP); 30% of UCB was bound even at low, premice...

Extraction of unconjugated bilirubin from serum.

Does conjugated bilirubin displace unconjugated bilirubin from albumin.